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KMID : 0380019940090020127
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 2 p.127 ~ p.131
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Production of Fructooligosaccharides by an Amyloglucosidase
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Abstract
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A new method of fructooligosaccharides production was investigated by an amyloglucosidase using sucrose as a substrate. Optimum reaction conditions were as follows : sucrose concentration, 700g/ ~ ; pH 5.5; temperature, 55C; enzyme dosage, 48 units per gram sucrose. At the optimized reaction conditions, 41.5% of fructooligosaccharides were produced after 25 hours. A hydrolyzing activity was stronger than transfructosylting activity at low sucrose concentrations, resulting in low production rate of fructooligosaccharides. The optimum pH and temperature in both transfructosylating(pH 5.5, 60°C ) and hydrolyzing activity(pH 4.75°C )were significantly different from each other. The amyloglucosidase also utilized fructooligosaccharides as a substrate and glucose seemed to be an inhibitor.
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